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目的探讨维生素D受体(VDR)的基因多态性与中国人的肺鳞癌易感性及其对药物化疗疗效的影响。方法选取广西柳州市工人医院2010年8月~2014年1月收治的肺鳞癌患者500例作为观察组,另选择自愿加入本研究的健康者200例作为对照组,采用聚合酶链反应(PCR)技术检测2组VDR基因型。比较2组基因型比例和不同基因型患者对化疗疗效的差异,分析基因多态性与肺鳞癌易感性的相关。结果观察组和对照组bb型基因分别占70.60%和49.00%,差异有统计学意义(χ2=3.859,P<0.05)。观察组有效Bsm1位点bb、Bb基因型分布频率分别为78.15%、21.85%,无效分别为86.86%、13.14%,2组间差异有统计学意义(χ2=3.729,P<0.05)。Apa1位点等位基因的分布对肺鳞癌的发生和治疗无明显影响。结论bb基因型患者在危险因素暴露的情况下更易患肺癌,同时对化疗药物的反应性也较差。
Objective To investigate the genetic polymorphism of vitamin D receptor (VDR) and susceptibility to lung squamous cell carcinoma in Chinese and its effect on the curative effect of chemotherapy. Methods 500 patients with squamous cell carcinoma of the lung in Liuzhou Workers’ Hospital of Guangxi Zhuang Autonomous Region from August 2010 to January 2014 were selected as the observation group. Another 200 healthy volunteers who were willing to participate in the study were selected as the control group. Polymerase chain reaction ) Technique to detect two groups of VDR genotypes. The differences between the two genotypes and the patients with different genotypes in the response to chemotherapy were compared, and the correlation between gene polymorphisms and lung squamous cell carcinoma was analyzed. Results The bb gene in observation group and control group accounted for 70.60% and 49.00% respectively, with significant difference (χ2 = 3.859, P <0.05). The frequencies of bb and bb genotypes in the effective Bsm1 site in the observation group were 78.15% and 21.85%, respectively, with the differences being 86.86% and 13.14%, respectively. The difference between the two groups was statistically significant (χ2 = 3.729, P <0.05). Apa1 locus allele distribution of lung squamous cell carcinoma of the occurrence and treatment had no significant effect. Conclusions Patients with bb genotype are more susceptible to lung cancer with exposure to risk factors and are less responsive to chemotherapeutic agents.