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目的观察糖尿病胃瘫大鼠胃窦平滑肌自噬相关蛋白Beclin-1和LC3的变化,探讨其在糖尿病胃动力障碍发生过程中的意义。方法将大鼠分为模型2周(DM2w)、模型4周(DM4w)和正常对照(NC)组,检测各组胃窦平滑肌Beclin-1和LC3蛋白表达。结果与NC组相比,DM2w、DM4w组胃窦平滑肌Beclin-1蛋白相对表达量均较高[(0.13±0.04)vs(0.22±0.12),P<0.05;(0.13±0.04)vs(0.62±0.07),P<0.01],且DM4w组高于DM2w组[(0.62±0.07)vs(0.22±0.12),P<0.01]。LC3蛋白显示LC3-Ⅰ和LC3-Ⅱ两条带,与NC组相比,DM2w、DM4w组胃窦平滑肌LC3-Ⅱ/LC3-Ⅰ比值均较高[(0.98±0.09)vs(2.28±0.20),P<0.01;(0.98±0.09)vs(2.34±0.21),P<0.01],DM2w组与DM4w组比较差异无统计学意义[(2.28±0.20)vs(2.34±0.21),P>0.05]。结论自噬相关蛋白Beclin-1和LC3的表达在糖尿病胃动力障碍发病前随病程逐渐加强,自噬可能影响糖尿病胃动力障碍发生。
Objective To observe the changes of the autophagy-related protein Beclin-1 and LC3 in the gastric antral smooth muscle of diabetic rats with gastric paralysis and to explore its significance in the pathogenesis of diabetic gastric motility disorders. Methods The rats were divided into DM2w for 2 weeks, DM4w for 4 weeks, and normal control group (NC). The expressions of Beclin-1 and LC3 in antral smooth muscle of each group were detected. Results Compared with NC group, the relative expression of Beclin-1 protein in gastric smooth muscle in DM2w and DM4w groups was significantly higher than that in NC group [(0.13 ± 0.04 vs 0.22 ± 0.12, P <0.05; 0.13 ± 0.04 vs. 0.62 ± 0.07), P <0.01], and DM4w group was higher than DM2w group [(0.62 ± 0.07) vs (0.22 ± 0.12), P <0.01]. Compared with NC group, the LC3-Ⅱ / LC3-Ⅰ ratios of LC3-Ⅱ / LC3-Ⅰ of gastric smooth muscle in DM2w and DM4w groups were significantly higher than those in NC group [(0.98 ± 0.09) vs (2.28 ± 0.20) , P <0.01; (0.98 ± 0.09) vs (2.34 ± 0.21), P <0.01]. There was no significant difference between DM2w group and DM4w group (2.28 ± 0.20 vs 2.34 ± 0.21, P 0.05) . Conclusions The expressions of autophagy-related proteins Beclin-1 and LC3 gradually increase with the progression of diabetes before the onset of gastric motility disorders. Autophagy may affect the occurrence of gastric motility disorders.