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为了研究常乐稀土多元复合肥对主要农作物--玉米(Zea mays L.)是否产生细胞毒性和遗传毒性效应,采用不同梯度质量浓度的稀土复合肥溶液对玉米进行处理,观察其根尖生长状况,统计分析细胞死亡率、微核率、有丝分裂指数以及对细胞DNA的损伤情况.结果表明,质量浓度为10mg·L-1以下时,常乐稀土复合肥能够促进玉米根尖的生长,100mg·L-1以上时抑制根尖生长;随着试验浓度的递增,细胞死亡率、微核率、DNA损伤率逐渐上升并具有正相关性,表现出明显的剂量-效应关系,而有丝分裂指数却随着浓度的增加逐渐下降.以上结果表明,常乐稀土复合肥对玉米确有一定的细胞毒性和遗传毒性效应,其细胞毒性阈值和遗传毒性阈值皆为100mg·L-1.
In order to study whether Changle REE could induce cytotoxicity and genotoxic effect on maize (Zea mays L.), the maize was treated with different concentrations of rare earth compound fertilizer solution, and the root tip growth status , The cell death rate, micronucleus rate, mitosis index and DNA damage were statistically analyzed.The results showed that when the mass concentration was below 10 mg · L-1, Changle rare earth compound fertilizer could promote the growth of corn root tip, 100mg · L-1 inhibited the growth of root tips. With the increase of test concentration, cell death rate, micronucleus rate and DNA damage rate increased gradually and positively correlated with dose-effect relationship, while mitotic index With the increase of concentration gradually decreased.The above results show that Changle rare earth compound fertilizer does have certain cytotoxic and genotoxic effects on maize, and its cytotoxicity threshold and genotoxicity threshold are 100mg · L-1.