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目的了解Ki-ras点突变在胃癌发生发展中的作用,对胃癌组织K-ras点突变进行分析。方法采用多聚酶链延伸反应-限制性片段长度多态性分析法(PCR-RFLP)对69例福尔马林液固定、石蜡包埋胃癌组织Ki-ras第12位密码子点突变作了检测,并对点突变与肿瘤生物学行为的关系进行分析。结果胃癌Ki-ras第12位密码子点突变率为7.2%。肠型胃癌为13.9%(5/36),显著高于弥漫型胃癌(0/33,P<0.05)。点突变的发生与肿瘤大小、浆膜浸润、淋巴结转移、临床分期及术后生存期无关。结论 Ki-ras第12位密码子点突变与肠型胃癌的发生发展有关。
Objective To understand the role of Ki-ras point mutation in the development of gastric cancer and to analyze the K-ras point mutation in gastric cancer. Methods Polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) was used to detect mutations in the Qi-ras codon 12 of formalin-fixed and paraffin-embedded gastric cancer tissues. The relationship between point mutations and tumor biological behavior was analyzed. Results The mutation rate of Ki-ras codon 12 in gastric cancer was 7.2%. Intestinal gastric cancer was 13.9% (5/36), significantly higher than diffuse gastric cancer (0/33, P<0.05). The occurrence of point mutations was not associated with tumor size, serosal infiltration, lymph node metastasis, clinical stage, and postoperative survival. Conclusion The point mutation of Ki-ras codon 12 is related to the occurrence and development of intestinal type gastric cancer.