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检测籼稻骨干亲本的稻瘟病抗性基因Pi-b,以期为抗病育种提供适合的亲本。以92份骨干亲本为材料,采用功能基因分子标记检测92份骨干亲本的Pi-b基因。结果表明,92份供试材料中,19份携带Pi-b基因,占供试材料20.65%。Pi-b基因在恢复系和不育系中的分布存在差异,81份恢复系中有19份携带该基因,占23.46%,11份不育系中没有检测到Pi-b基因,恢复系携带Pi-b基因比例远高于不育系。Pi-b基因在各稻区的材料都有分布,来自福建三明市、四川省、陕西省和湖南岳阳市农业科学研究所的材料分别有1份、9份、7份和1份携带Pi-b基因。
The blast resistance gene Pi-b of indica main parents was detected to provide suitable parents for resistance breeding. 92 parents were used as materials to detect the Pi-b gene of 92 parents by functional gene molecular markers. The results showed that 19 out of 92 tested materials carried Pi-b gene accounting for 20.65% of the tested materials. There were differences in the distribution of Pi-b gene between restorer lines and CMS lines, of which, 19 of 81 restorer lines carried this gene, accounting for 23.46%. Pi-b gene was not found in 11 CMS lines, Pi-b gene is much higher than the male sterile line. The Pi-b gene was distributed in all rice regions. The materials from Sanming City in Fujian Province, Sichuan Province, Shaanxi Province and Yueyang Institute of Agricultural Sciences in Hunan Province were 1, 9, 7 and 1 respectively. b gene.