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目的 分析中暑与对照士兵外周血淋巴细胞热应激蛋白 71(HSP71)基础和诱导性水平的差异 ,探讨中暑发生的分子机制。方法 采用流式细胞术方法检测两组士兵外周血淋巴细胞HSP71。结果 基础状态下 ,对照和中暑士兵HSP71水平 (对数平均荧光强度分别为 2 6 8.10、390 .0 5 )均较低 ,且差异无显著性 (P >0 .0 5 ) ;在体外 37℃培养条件下 ,淋巴细胞HSP71水平较基础状态明显上升 ,中暑士兵HSP71水平 (对数平均荧光强度为 140 5 .0 8)在此条件下较对照士兵 (对数平均荧光强度为 931.16 )更高 ,差异有显著性 (P <0 .0 1) ;在 41℃应激 1h后 ,对照组士兵淋巴细胞HSP71平均水平轻度升高 (对数平均荧光强度为 95 5 .99) ,而中暑组HSP71不但没有升高 ,反而降低 (对数平均荧光强度为 1148.92 )。结论 中暑士兵外周血淋巴细胞HSP71诱导性发生改变 ,提示中暑士兵HSP71基因及其表达存在差异性 ,其热耐阈下降 ,热耐受能力不能正常形成。
Objective To analyze the differences of basic and inducible levels of heat shock protein 71 (HSP71) in peripheral blood lymphocytes between stroke and control subjects and to explore the molecular mechanism of heat stroke. Methods Flow cytometry was used to detect the peripheral blood lymphocyte HSP71 in two groups of soldiers. Results Under the basal condition, the level of HSP71 (logarithmic mean fluorescence intensity was 26.88, 1090.050, respectively) was lower in control and heat stroke soldiers with no significant difference (P> 0.05) Under the conditions of culture, the level of HSP71 in lymphocytes increased significantly compared with the baseline, and the level of HSP71 (logarithmic mean fluorescence intensity was 140 5 .0 8) was higher than that of the control soldiers (mean logarithmic fluorescence intensity was 931.16) The difference was significant (P <0.01). After being stressed at 41 ℃ for 1h, the average level of HSP71 in the control group increased slightly (logarithmic average fluorescence intensity was 95.599) Not only did not increase, but lower (log average fluorescence intensity of 1148.92). Conclusions The changes of HSP71 in peripheral blood lymphocytes in stroke sufferers are different. It suggests that HSP71 gene and its expression are different in heat stroke soldiers, and its heat tolerance threshold is decreased and heat tolerance is not formed normally.