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关于抗鼠弓形虫的单克隆抗体已有报道。单克隆抗体B在人补体存在下具有杀鼠弓形虫的作用,其相应抗原为分子量约为30,000的虫表面蛋白P30。作者利用这一特性提取纯化虫表面蛋白P30。先将CNBr琼脂糖蛋白A在0.01M HCl中溶胀,在一定条件下与单克隆抗体B混合,4℃放置过夜,使该抗体结合于琼脂糖蛋白A之上,经充分洗涤并用甘氨酸将未被结合的活性基团饱和后,便可用来作为免疫吸附剂。虫体用表面活性剂Nonidet P40处理,提出虫抗原蛋白质,然后在室温下与前面所得免疫吸附剂混合,反应2.5小时,单克隆抗体B所抗的抗原蛋白P30即被吸附于琼脂糖上,经充分洗涤
Monoclonal antibodies against Toxoplasma gondii have been reported. Monoclonal antibody B has the function of killing Toxoplasma gondii in the presence of human complement, and the corresponding antigen is insect surface protein P30 with a molecular weight of about 30,000. The authors use this feature to extract the purified pest surface protein P30. CNBr agarose A was first swollen in 0.01 M HCl, mixed with monoclonal antibody B under certain conditions and left overnight at 4 ° C to allow the antibody to bind to agarose A, washed well with glycine and not After the bound active group is saturated, it can be used as an immunoadsorbent. The parasites were treated with surfactant Nonidet P40 to prepare the pest antigen protein and then mixed with the previously obtained immunosorbent at room temperature for 2.5 hours. The antigen protein P30 against the monoclonal antibody B was adsorbed onto agarose Wash well