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本文根据单克隆抗体夹心法检测的原理建立了一种新的单抗夹心微球免疫荧光法,并用于肿瘤患者胸腹水的定性分析,为临床恶性浆膜炎的诊断和鉴别提供了一种新的检测方法。 材料和方法 一、单克隆抗体的纯化及免疫吸附微球的制备:取含鼠抗人胃癌单抗的小鼠腹水(本室制备),经50％饱和硫酸铵盐析沉淀,透析后上DEAE-52纤维素柱(φ2.5×25cm),洗脱未吸附物质至OD280nm<0.01后用NaCl梯度洗脱,收集IgG峰。获得纯化的MG7,MGd1单抗按1:1混合,制成免疫吸附
In this paper, based on the principle of monoclonal antibody sandwich assay to establish a new monoclonal antibody sandwich microsphere immunofluorescence, and for the analysis of pleural effusion in patients with cancer qualitative analysis for the clinical diagnosis and differential diagnosis of malignant seromas provides a new The detection method. MATERIALS AND METHODS A. Purification of Monoclonal Antibody and Preparation of Immunosorbent Microspheres: Ascites (prepared in our laboratory) from mice containing anti-human gastric cancer monoclonal antibody was precipitated by 50% saturated ammonium sulfate and dialyzed on a DEAE -52 cellulose column (φ2.5 × 25cm), eluting non-adsorbed material until OD280nm <0.01 and eluting with NaCl gradient to collect IgG peak. Obtained purified MG7, MGd1 monoclonal antibody by 1: 1, made by immunosorbent