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实验动物为Slc:Donryu系雄性大鼠(150～170g)以及Sic:Wistar系雄性大鼠(180～200g)。在温度23±2C,湿度50%～50%,明暗(12h/12h)交替饲养室预饲养1周后供实验用。试样的制备:①C5-ext:组成堵苓汤的5味生药(猪苓、茯苓、泽泻、滑石、阿胶)等量以10倍水加热提取2小时,过滤后残渣再次提取,两次滤液合之冷冻干燥(得率18.9%)。②C4+1-ext:除去阿胶的其余4味生药以10倍水2小时2次加热提取,滤液中添加阿胶的热水提取物溶解后冷冻干燥(20.0%)。③C4-ext:除阿胶外其余4味生药
The experimental animals were Slc:Donryu male rats (150-170 g) and Sic: Wistar male rats (180-200 g). In the temperature 23 ± 2C, humidity 50% ~ 50%, light and dark (12h/12h) alternate breeding room pre-feeding for 1 week for experimental use. Preparation of the sample: 1C5-ext: 5-flavored crude drugs (brachneus, cockroach, rhynchophylla, talcum, and ejiao) composing the blocking soup are heated and extracted in 10 times the water for 2 hours. The residue is filtered and the filtrate is extracted twice. Combined freeze-drying (yield 18.9%). 2C4+1-ext: The remaining 4 herbs of the donkey-hide type were extracted by heat extraction with 10 times water for 2 hours, and the hot water extract containing the gelatin was dissolved in the filtrate and freeze-dried (20.0%). 3C4-ext: 4 other herbs except ejiao