论文部分内容阅读
目的:检测转化生长因子 TGF-β/smad 信号通路相关蛋白在高氧肺损伤中的表达,探讨其与基因 LKB1表达的相关性.方法:40只3日龄新生大鼠依据是否高浓度氧暴露随机分为二组:正常对照组(空气环境下暴露7天)和高氧组(95%氧暴露7天);实验结束后留取肺组织标本,光镜观察并盲法评分,免疫组化法检测肺组织 LKB1、TGF-β1、psmad2蛋白的表达和分布,比较两种组织表达程度的差异.结果:与正常对照组相比,高氧组中的 LKB1、TGF-β1、psmad2蛋白均有降低,LKB1、TGF-β1蛋白表达差异有显著性 P<0.05,psmad2蛋白表达差异无显著性,高氧组肺组织中,LKB1与 TGF-β1蛋白表达呈正相关,有统计学意义(r=0.474,P=0.04),TGF-β1与 psmad2蛋白表达呈正相关,有统计学意义(r=0.491,P=0.033).结论:高氧肺损伤中,TGF-β/smad 信号通路可能受到 LKB1的分子调控,TGF-β/smad 信号通路可能参与高氧导致的肺损伤发生.“,”Objective To detect the expression of important proteins associated with TGF-β/Smad signaling pathway in lung of newborn rats of hyperoxia-exposed and investigate the correlation of these proteins to LKB1 gene expression. Methods 40 3-day-old SD rats from two litters were randomly divided into two groups as hyperoxia-exposed group(A),and air-exposed group(B).The rats from the group A were placed in a sealed Plexiglas chamber with a minimal in-and outflow, providing six to seven exchanges per hour of the chamber volume and maintaining O2 levels above 95%.while the rats in the group B only exposed with air simultaneously. Seven days later, al of the rats were sacrificed. The expression and localization of LKB1,TGF-β1 and pSmad2 proteins were detected with immunohistochemicalstaining. Results The expressions of LKB1,TGF-β1and pSmad2 were lower in and the differences in LKB1 and TGF-βproteinswere. Significantly different between them(P<0.05). In PJSpolyps positive correlations were found between LKB1 and TGF-β1 and Between TGF-β1 and pSmad2 expressions. Conclusion TGF-β/Smad pathway is probably subjected to there gulation by LKB1. And may play a role in the occurrence of hyperoxia-induced lung damage.