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通过克隆得到斑节对虾(Penaeus monodon)组织蛋白酶L基因(PmCatL)cDNA全长。该基因序列全长1 850bp,包括1 026 bp的开放阅读框,编码341个氨基酸。预测的PmCatL蛋白由信号肽(Met~1-Ala~(18))、前体域(Val~(19)-Gln~(123))和成熟域(Leu~(124)-Val~(341))三部分构成,存在3个半胱氨酸蛋白酶活性位点(Cys~(148)、His~(287)、Asn~(308))和1个潜在的糖基化位点(Asn~(99))。具有组织蛋白酶L的E~(45)X_3RX_3FX_2NX_3IX_3N~(64)和G~(187)CXGG~(192)保守序列。同源性分析显示,PmCatL氨基酸序列与其他物种相似性为36%~75%。进化树显示PmCatL和罗氏沼虾(Macrobrachium rosenbergii)亲缘关系最近,并与其他无脊椎动物的组织蛋白酶L聚成一支。表达结果表明,PmCatL基因在肝胰腺、淋巴、肌肉、性腺等被测组织中均有表达,其中淋巴和肝胰腺的表达量较高;PmCatL基因在斑节对虾仔虾时期表达量较高,可能与斑节对虾在这一时期的生长发育相关;PmCatL在卵巢发育的Ⅳ期表达量最高,可能在斑节对虾的卵巢发育中发挥重要作用。
The whole length of PmCatL cDNA of Penaeus monodon was cloned. The full length of this gene is 1 850 bp, including an open reading frame of 1026 bp, encoding 341 amino acids. The predicted PmCatL protein consists of the signal peptide (Met ~ 1-Ala ~ (18)), the precursor domain (Val ~ (19) ~ Gln ~ 123) and the mature domain (Leu ~ (124) (Cys 148, His 287, Asn 308) and one potential glycosylation site Asn 99 )). E45X_3RX_3FX_2NX_3IX_3N ~ (64) and G ~ (187) CXGG ~ (192) conserved sequences with cathepsin L. Homology analysis showed that the similarity of PmCatL amino acid sequence with other species was 36% -75%. Phylogenetic tree showed that PmCatL is closest to Macrobrachium rosenbergii and integrates with cathepsin L of other invertebrates. The expression results showed that the PmCatL gene was expressed in the liver, pancreas, lymph, muscle, gonad and other tissues, and the expression of PmCatL gene was higher in lymphatic and hepatopancreas. Which was related to the growth and development of Penaeus monodon in this period. PmCatL expressed highest in stage Ⅳ of ovary development and may play an important role in ovary development of Penaeus monodon.