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OBJECTIVE: Geophila repens(L.) I. M. Johnst.(Rubiaceae), a small, creeping, perennial herb, is claimed to have memory-enhancing property. The goal of this study was to assess its antioxidant and anticholinesterase activity and conduct a rapid bioautographic enzyme assay for screening acetylcholinesterase(ACh E) and butyrylcholinesterase(BCh E) inhibition of G. repens extracts.METHODS: Antioxidant activity of G. repens extracts was assessed by performing 1,1-diphenyl-2-picrylhydrazyl(DPPH), nitric oxide(NO), superoxide(SOD), hydroxyl(OH) and total antioxidant capacity(TAC) assays. Anticholinesterase activity was investigated by quantifying the ACh E and BCh E inhibitory activities of chloroform(CGR), ethyl acetate(EGR) and methanol(MGR) extract fractions from G. repens leaves. A rapid high-performance thin-layer chromatography(HPTLC) bioautographic method for the detection of ACh E and BCh E inhibition was performed.RESULTS: Among all extract fractions, EGR exhibited the highest half maximal inhibitory concentration(IC_(50)) in DPPH, SOD, NO, OH and TAC assays, with IC_(50) of(38.33 ± 3.21),(45.14 ± 1.78),(59.81 ± 1.32),(39.45 ± 0.79) and(43.76 ± 0.81) μg/m L respectively. EGR displayed competitive, reversible inhibition of ACh E and BCh E activities with IC_(50) of(68.63 ± 0.45) and(59.45 ± 0.45) μg/m L, respectively. Total phenolic and flavonoids contents of EGR were found to be 360.42 mg gallic acid equivalents and 257.31 mg quercetin equivalents per gram of extract. Phytoconstituents of the EGR extract that were inhibitors of cholinesterase produced white spots on the yellow background of HPTLC plates in the bioautographic test.CONCLUSION: The results of this study revealed that phenols and flavonoids could be responsible for the antioxidant, anticholinesterase activities of G. repens.
OBJECTIVE: Geophila repens (L.) IM Johnst. (Rubiaceae), a small, creeping, perennial herb, is claimed to have memory-enhancing property. The goal of this study was to assess its antioxidant and anticholinesterase activity and conduct a rapid bioautographic enzyme assay for screening acetylcholinesterase (ACh E) and butyrylcholinesterase (BCh E) inhibition of G. repens extracts. METHODS: Antioxidant activity of G. repens extracts was assessed by performing 1,1-diphenyl- 2-picrylhydrazyl (DPPH) (NO), superoxide (SOD), hydroxyl (OH) and total antioxidant capacity (TAC) assays. Anticholinesterase activity was investigated by quantifying the ACh E and BCh E inhibitory activities of chloroform (CGR), ethyl acetate MGR) extract fractions from G. repens leaves. A rapid high-performance thin-layer chromatography (HPTLC) bioautographic method for the detection of ACh E and BCh E inhibition was performed .RESULTS: Among all extract fractions, EGR exhibited the highest half ma (50) of 38.33 ± 3.21, (45.14 ± 1.78), (59.81 ± 1.32), (39.45 ± 0.79) × DPPH, SOD, NO, OH and TAC assays, and (43.76 ± 0.81) μg / m L respectively. EGR displayed competitive, reversible inhibition of ACh E and BCh E activities with IC 50 (68.63 ± 0.45) and (59.45 ± 0.45) μg / m L, respectively. phenolic and flavonoids contents of EGR were found to be 360.42 mg gallic acid equivalents and 257.31 mg quercetin equivalents per gram of extract. Phytoconstituents of the EGR extract that were inhibitors of cholinesterase produced white spots on the yellow background of HPTLC plates in the bioautographic test. CONCLUSION: The results of this study revealed that phenols and flavonoids could be responsible for the antioxidant, anticholinesterase activities of G. repens.