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辐射诱发的DNA损伤和修复,不仅影响到多聚核苷酸链,对DNA的超螺旋也有作用.本文通过对大鼠胸腺(T)细胞和脾(S)细胞核质体粘度和沉降率的检测,比较了经X线照射后,T、S细胞DNA超螺旋的损伤和修复.实验用的T、S细胞取自雌性Sprague-Dawley大鼠,悬浮于无Ca~(++)、Mg~(++)的Hank’s液中(25×10~6细胞/ml).制备好的细胞悬液应尽快用于照射,辐照采用西门子710H X线机,剂量率为1.75Gy/min,一次照射剂量范围为0.6～19.2Gy,对照用模拟照射,研究修复现象的细胞照射后应在37℃下温浴适
Radiation-induced DNA damage and repair not only affects the polynucleotide chain, but also has a role in the supercoiling of DNA. In this paper, the visceral (T) cells and spleen (S) cell nucleoplast viscosities and sedimentation rates were measured. Comparing the damage and repair of DNA super-helices of T and S cells after X-ray irradiation. Experimental T and S cells were obtained from female Sprague-Dawley rats, suspended in no Ca~(++), Mg~( ++) in Hank’s solution (25 × 10 ~ 6 cells/ml). The prepared cell suspension should be used as soon as possible irradiation, irradiation using Siemens 710H X-ray machine, a dose rate of 1.75Gy/min, a single dose The range is 0.6 to 19.2 Gy, and the control is simulated irradiation. After the irradiation of the cells to repair the phenomenon, the temperature should be appropriate at 37°C.