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应用AFLP分析技术,比较了红莲型水稻雄性不育系和保持系线粒体DNA,找到一不育系特异条带TA1。以该条带DNA为探针与不育系、保持系以及F_1杂种线粒体总RNA杂交,结果显示该片段黄化苗期三者均转录一个RNA分子,但转录产物分子量各不相同,说明三者该片段阅读框架不同。经测序,该片段长202bp,序列内含密码子ATG、ATT、AGA、AGG以及正向重复序列5’TGTAC3’、5’ATTATTTT3’和倒转重复序列5’GGGAAACA3’。上述特点表明该片段可能是某一蛋白编码序列,并可能与红莲型水稻雄性不育性状形成有关。
AFLP analysis technique was used to compare the mitochondrial DNA of the HLK male sterile line and its maintainer line to find a TA1 specific band. The DNA of this band was used as a probe to hybridize with CMS lines and maintainer lines and F_1 hybrid mitochondrial total RNA. The results showed that all three RNAs were transcribed in the etiolated stage, but the molecular weights of the transcripts were different, The fragment reading frame is different. After sequencing, the fragment was 202 bp in length and contained codons ATG, ATT, AGA, AGG and forward repeats 5’TGTAC3 ’, 5’ATTATTTT3’ and inverted repeat 5’GGGAAACA3 ’. The above characteristics indicate that the fragment may be a protein coding sequence, and may be related to the formation of male sterile characters of HL.