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测定叶绿素a诱导荧光是研究光合作用的非常有效的手段,近年来在检测逆境因子对植物光合机构的影响方面得到广泛的应用.以前的研究主要是通过检测叶绿素a诱导荧光动力学的慢相部分来研究环境胁迫对光合机构的影响机理,如F_v/F_m(光系统II的光化学效率)、qP(光化学猝灭)和qN(非光化学猝灭)等是常用的指标.Melis通过观察叶绿素a诱导荧光动力学的快相部分发现,叶片的光系统Ⅱ(PSⅡ)有一部分反应中心不能将电子从Q_A传递到Q_B,这部分反应中心被称为Q_B-非还原中心(Q_B-nonreducing center)或失活的反应中心
Determination of chlorophyll a -induced fluorescence is a very effective means of studying photosynthesis, and in recent years it has been widely used in detecting the effects of adverse factors on plant photosynthetic machinery.Previous studies mainly focused on the detection of chlorophyll a -induced slow phase of fluorescence kinetics (Such as F_v / F_m (photochemical efficiency of photosystem II), qP (photochemical quenching) and qN (non-photochemical quenching) are all commonly used indexes to study the mechanism of environmental stress on photosynthetic mechanism.Melis was induced by observing chlorophyll a In the fast phase of fluorescence kinetics, some reaction centers in photosystem Ⅱ (PSⅡ) of leaves do not transfer electrons from Q_A to Q_B, which is called Q_B-nonreducing center or missing Live reaction center