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目的了解丽水市艾滋病病毒-1(HIV-1)阳性人群蛋白印迹试验(WB)的带型分布特征,为制定该市艾滋病防控措施提供科学依据。方法对于HIV确证阳性样本采用WB检测,结果严格按照试剂盒说明书判断,并用SPSS 16.0统计软件分析。结果 697例HIV-1确证阳性标本中,除p55和p17抗体出现率分别为42.49%(296例)和75.61%(527例)较低外,其余带型出现率均在92%以上;带型组合以全带和次全带为主(85.51%,596例)。不同性别中p17抗体出现率差异有统计学意义(χ~2=4.927,P=0.026);不同年龄组中p24抗体出现率差异有统计学意义(χ~2=7.998,P=0.046);不同性传播途径中p51和p17抗体出现率差异均有统计学意义(χ~2=8.384,P=0.004)和(χ~2=11.826,P=0.001);不同人群中p17抗体出现率差异有统计学意义(χ~2=13.71,P=0.033)。结论不同性别、人群和传播途径中,p17或p51抗体检出率存在显著性差异;p24抗体在60岁以上老年组丢失显著增多,说明该人群处于艾滋病期较多,应加强该人群监测力度。丽水市处于HIV感染增长快速期,对WB带型特征在不同人群中的分析,为该地区HIV疫情防控提供科学依据。
Objective To understand the band-type distribution of Western blot test (WB) of HIV-1 positive population in Lishui and provide a scientific basis for the development of AIDS prevention and control measures in this city. Methods WB positive HIV positive samples were detected by WB, the results in strict accordance with kit instructions to determine, and use SPSS 16.0 statistical software analysis. Results Among the 697 HIV-1 positive samples, the incidence rates of other bands were all above 92%, except for the low rates of p49 and p17 antibody being 42.49% (296 cases) and 75.61% (527 cases) The combination of the whole band and sub-full band (85.51%, 596 cases). The incidence of p17 antibody in different genders was significantly different (χ ~ 2 = 4.927, P = 0.026). The incidence of p24 antibody in different age groups was significantly different (χ ~ 2 = 7.998, P = 0.046) The incidences of p51 and p17 antibodies in sexually transmitted pathways were significantly different (χ ~ 2 = 8.384, P = 0.004) and (χ ~ 2 = 11.826, P = 0.001) Significance (χ ~ 2 = 13.71, P = 0.033). Conclusion There is a significant difference in the detection rate of p17 or p51 antibody among different genders, populations and routes of transmission. The loss of p24 antibody in the elderly over 60 years old is significantly increased, indicating that the population is in the AIDS stage and should be monitored intensively. Lishui city in the rapid growth of HIV infection, WB banding characteristics in different groups of people in the area for the prevention and control of HIV provide a scientific basis.