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目的:考察灌胃给药后葡萄内酯在小鼠体内各个组织中的分布情况。方法:给药后,于不同时间点取心、肝、脾、肺、肾、脑、胃、肠、肌肉、脂肪10个组织,制成组织匀浆,用乙酸乙酯萃取富集,氮吹处理后甲醇定容,采用高效液相色谱法测定并计算各个组织中葡萄内酯的浓度。结果:给药后30 min各组织中药物浓度由高到低依次为:胃>肠>肝脏>脂肪>肾>心脏>肌肉>肺>脾>脑;给药后120 min心、肝、脾、肾、脑等组织中出现分布高峰;480 min后依次消除。结论:葡萄内酯能被迅速吸收并广泛分布到各个组织,但吸收消化的过程比较持久,能透过血脑屏障,在靶器官中的作用时间>8 h。
Objective: To investigate the distribution of grape lactone in various tissues of mice after intragastric administration. METHODS: After administration, 10 tissues of liver, spleen, lung, kidney, brain, stomach, intestine, muscle and fat were taken at different time points to make tissue homogenate, extracted with ethyl acetate, After treatment, the volume of methanol was determined, and the concentration of grape lactone in each tissue was determined by high performance liquid chromatography. Results: The drug concentrations in the tissues from high to low were: stomach> intestine> liver> fat> kidney> heart> muscle> lung> spleen> brain at 30 min after administration. Heart, liver, Kidney, brain and other organizations appear peak distribution; 480 min followed by elimination. CONCLUSION: Volactone can be quickly absorbed and widely distributed in various tissues. However, the process of absorption and digestion persists and can penetrate the blood-brain barrier, and its action time in target organs is> 8 h.