论文部分内容阅读
目的:研究GPER(G protein-coupled estrogen receptor)在子宫内膜样腺癌(EEC)组织中的表达情况,探讨GPER对EEC临床病理因素、预后的意义及其与雌激素受体(ER)之间的相关性。方法:采用实时定量PCR技术检测EEC组织中的GPER mRNA含量,免疫组化法检测GPER蛋白含量,分析GPER表达对EEC的临床意义。结果:GPER高表达于FIGO晚期、低分化、子宫肌层深浸润及淋巴转移的EEC组织中。单因素分析结果显示,GPER mRNA高表达的患者预后较差(OS,P=0.001;DSS,P=0.001;PFS,P<0.001),GPER蛋白阳性的患者预后亦较差(OS,P=0.009;DSS,P=0.009;PFS,P<0.001)。多因素分析发现,GPER不是影响EEC患者预后的独立因子。GPER与ER无统计学相关性。结论:GPER不是EEC预后的独立因子,但在其临床生物学行为中扮演重要的角色。
OBJECTIVE: To study the expression of GPR (G protein-coupled estrogen receptor) in endometrioid adenocarcinoma (EEC) and to explore the clinicopathological factors and prognosis of GPEC and the relationship between GPER and estrogen receptor (ER) Relevance between. Methods: The contents of GPER mRNA in EEC tissues were detected by real-time quantitative PCR. The content of GPER protein was detected by immunohistochemistry. The clinical significance of GPER expression in EEC was analyzed. Results: GPER was highly expressed in advanced, poorly differentiated, deep myometrial invasion and lymphatic metastasis of EEC tissues. The results of univariate analysis showed that the patients with high GPER mRNA expression had a poor prognosis (OS, P = 0.001; DSS, P = 0.001; PFS, P <0.001) ; DSS, P = 0.009; PFS, P <0.001). Multivariate analysis found that GPER was not an independent factor affecting the prognosis of EEC patients. GPER and ER no statistical correlation. Conclusion: GPER is not an independent factor of EEC prognosis, but plays an important role in its clinical biological behavior.