论文部分内容阅读
预先给与苯巴比妥的大鼠空腹接触二硫化碳(CS_2)24小时后,组织学上出现小叶中心损害,并伴有肝脏化学及生化方面的改变(含水量增多,细胞色素P-450减少);而在接触CS_2前,预先未给与苯巴比妥的大鼠,未发现小叶中心损害,仅见细胞色素P-450略为减少。给苯巴比妥的动物,~(14)CS_2较大程度地转为~(14)CO_2。体外试验指出,只有当NADPH存在时,CS_2才可能引起细胞色素P-450的显著下降。这一发现及观察结果提示:CS_2需经代谢才对肝脏有损害。目前进行对CS_2的代谢和在控制条件下CS_2引起细胞色素F-450破坏的体外研究。用~(14)C和~985)S标记的CS_2试验发现,在CS_2破坏P-450的情况下,CS_2被氧化脱硫,脱下的部分硫成为共价结合作用于微粒
Rats exposed to phenobarbital with fasting exposure to carbon disulfide (CS_2) for 24 hours histologically developed centrilobular damage accompanied by chemical and biochemical liver changes (increased water content and reduced cytochrome P-450) ; While in rats exposed to phenobarbital before CS 2 exposure, no centrilobular damage was found, with only a slight decrease in cytochrome P-450. To phenobarbital animals, ~ (14) CS_2 was largely converted to ~ (14) CO_2. In vitro experiments indicate that CS 2 may cause a significant decrease in cytochrome P-450 only in the presence of NADPH. The findings and observations suggest that CS 2 is metabolized to damage the liver. In vitro studies of the metabolism of CS2 and the destruction of cytochrome F-450 by CS2 under controlled conditions are currently underway. The results of CS_2 labeled with ~ (14) C and ~ 985) S showed that under the condition of CS_2 destroying P-450, CS_2 was oxidatively desulfurized and part of the sulfur removed became covalently bound to the microparticles