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在实验室建立了分散的新生大鼠海马神经细胞体外培养模型。该模型属原代培养，在体外能直接观察神经元的生长发育。研究表明：该模型稳定性好，差异性小；培养神经细胞可存活１个月以上，１４ｄ左右细胞生长最为丰满，且四周晕光明显，神经突起多而粗大，分枝互成网络；１４ｄ以后生长减缓。由于在此模型培养的条件下，既不存在血脑屏障问题，又容易控制条件，便于在细胞和分子水平上深入研究神经细胞的形态学、生理学、生化代谢及药理学等方面的问题，特别适合在体外进行神经药理学的研究。
An in vitro model of cultured neonatal rat hippocampal neurons was established in the laboratory. The model is a primary culture, in vitro can directly observe the growth and development of neurons. The results showed that the model has good stability and little difference. The cultured neurons survived for more than 1 month, the cells grew more abundant on the 14th day, Slow down. Because of the absence of a blood-brain barrier problem and easy control of the conditions under which this model is cultivated, it facilitates further study of the neuronal morphology, physiology, biochemical metabolism and pharmacology at the cellular and molecular level, in particular Suitable for in vitro neuropharmacology research.