参考Maehly和Chance法,研究出一种简便的过氧化物酶定量测定方法。该方法采用愈创木酚及过氧化氢为底物,根据产物四邻甲氧基联酚的消光系数利用公式求出过氧化物酶的活性单位。经与标准辣根过氧化物酶的对照。证明该方法一分钟测定值准确性、重复性可靠。采用去垢剂制备组织匀浆测得的过氧化物酶活性明显高于水制匀浆。该方法简便快速,最大优点是能测定组织的过氧化物酶活性,为临床诊断及科研提供一种实用的方法。 With reference to Maehly and Chance methods, a simple method for quantitative determination of peroxidase was developed. The method uses guaiacol and hydrogen peroxide as substrates, and uses the formula to calculate the peroxidase activity unit according to the extinction coefficient of the product o-methoxydiphenol. After comparison with standard horseradish peroxidase. Proved that the method of one minute measurement accuracy, reproducibility and reliability. Peroxidase activity was significantly higher in tissue homogenates prepared using detergents than in aqueous homogenates. The method is simple and rapid, and the biggest advantage is that it can measure the tissue peroxidase activity and provide a practical method for clinical diagnosis and research.