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选取4份耐低温弱光性不同的辣椒品种,以耐低温弱光指数作为品种耐性鉴定的依据,研究了四叶一心期,昼/夜温度10℃/5℃,光照70μmol·m-2·s-1的低温弱光下叶片叶绿素荧光特性,分析了其与耐低温弱光指数的关系。结果表明,低温弱光处理后,辣椒叶片的初始荧光(Fo)、最大荧光(Fm)、光合系统Ⅱ(PSⅡ)最大光化学量子产量(Fv/Fm)和光化学淬灭系数(qP)呈降低趋势;非光化学淬灭系数(q N)在处理3和5 d显著上升,处理7 d,非耐性品种qN显著下降,耐性品种qN显著上升或无显著变化;最大相对电子传递速率(rETRmax)和快速光响应曲线初始斜率(α)呈下降趋势;半饱和光强(Ik)在非耐性品种中呈下降趋势,但在耐性品种中呈上升趋势;随着光照强度不断增强,qP不断下降,但耐性品种发生完全光抑制的光强显著大于非耐性品种。Fo、Fm、qP、qN、Fv/Fm、rETRmax、α、Ik等叶绿素荧光参数与耐低温弱光指数显著相关,其中r ETRmax最稳定灵敏。qP光响应曲线和r ETRmax可作为实际生产中辣椒耐低温弱光的主要筛选指标。
Four low-temperature and poor light-poor varieties of pepper were selected to tolerate the low temperature and weak light index as the basis for the identification of tolerance to four-leaf one-heart, day / night temperature 10 ℃ / 5 ℃, light 70μmol · m-2 · s-1 under low temperature and low light, the relationship between chlorophyll fluorescence and low temperature resistant and weak light index was analyzed. The results showed that initial fluorescence (Fo), maximum fluorescence (Fm), maximum photochemical quantum yield (Fv / Fm) and photochemical quenching coefficient (qP) of photosynthetic system Ⅱ decreased with the treatment of low temperature and poor light (QN) increased significantly at 3 and 5 d after treatment, and the qN of non-tolerance varieties decreased significantly at the 7th day after treatment, while qN increased significantly or no significant change. The maximum relative electron transfer rate (rETRmax) and fast The initial slope of the light response curve (α) showed a downward trend; the half-saturation light intensity (Ik) showed a downward trend in non-tolerant varieties but increased in resistant varieties; qP decreased continuously with increasing light intensity, but the tolerance Light intensity of complete photoinhibition was significantly greater in varieties than in non-tolerance varieties. The chlorophyll fluorescence parameters such as Fo, Fm, qP, qN, Fv / Fm, rETRmax, α and Ik were significantly correlated with low temperature and weak light index, among which r ETRmax was the most stable and sensitive. qP light response curve and r ETRmax can be used as the actual production of pepper low temperature weak light the main screening indicators.