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目的研究知母有效成分(化合物9714)对拟血管性痴呆(VD)模型大鼠学习记忆的影响及其作用机制。方法采用双侧颈总动脉结扎(Commoncarotidarteryocclusion,CCAO)致拟VD模型大鼠,连续给药40d,以Morris水迷宫检测大鼠学习记忆能力,以免疫组化法检测大鼠脑皮质BDNF、ICAM1、VCAM1的表达。结果化合物971410、20、40mg·kg-1组能明显缩短定位航行试验中d2、d3的逃避潜伏期,提高空间搜索试验中原平台象限游泳距离占总距离的百分比(P<0.05,P<0.01);20、40mg·kg-1组能明显增加模型动物脑皮质BDNF的表达,降低脑皮质ICAM1的表达(P<0.05,P<0.01)。结论化合物9714能提高拟VD大鼠的学习记忆能力,同时对缺血后的神经元损伤、炎性损伤具有一定的保护作用。
OBJECTIVE: To investigate the effect of anemarrhenae active compound (compound 9714) on learning and memory in a rat model of vascular dementia (VD) and its mechanism. METHODS: Rats with VD were induced by common carotid artery ligation (CCAO) and administered continuously for 40 days. Morris water maze was used to test the learning and memory abilities of rats. Immunohistochemistry was used to detect BDNF and ICAM1 in rat cerebral cortex. Expression of VCAM1. Results Compounds 971410, 20 and 40 mg·kg-1 significantly reduced the escape latency of d2 and d3 in the navigation experiment, and increased the percentage of swimming distance in the original platform quadrant in the spatial search experiment (P<0.05, P<0.01). The 20 and 40 mg·kg-1 groups significantly increased the expression of BDNF in cerebral cortex of model animals and decreased the expression of ICAM1 in cerebral cortex (P<0.05, P<0.01). Conclusion Compound 9714 can improve the learning and memory abilities of quasi-VD rats, and it also has a protective effect on neuronal damage and inflammatory injury after ischemia.