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建立人血浆中亚胺培南浓度的高效液相色谱测定方法，探讨亚胺培南人体内药动学过程。以０．５ｍｏｌ／ＬＮ－２－羟乙基哌嗪－Ｎ－２－乙磺酸—５０％乙二醇（体积比１１）为稳定剂，以５－羟基－３－吲哚乙酸为内标，在μ－ＢｏｎｄａｐａｋＣ１８柱上，以０．３ｍｏｌ／Ｌ四乙基溴化铵乙醇液－２０ｍｍｏｌ／Ｌ磷酸氢二钾（体积比４９６）为流动相，检测波长２９８ｎｍ，对亚胺培南进行定量测定。结果血浆中亚胺培南在１～１００μｇ／ｍｌ浓度范围内具良好线性关系，血浆回收率为９８．６％±２．２５％（ｎ＝６）。血浆日内及日间ＲＳＤ分别为１．６９％～３．７６％和３．８２％～６．６７％。本方法简便、快捷、灵敏、准确；适用于临床血药浓度测定。
To establish a method for the determination of imipenem in human plasma by high performance liquid chromatography and to investigate the pharmacokinetics of imipenem in human. Using 0.5mol / LN-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid-50% ethylene glycol (volume ratio 11) as stabilizer and 5-hydroxy-3-indoleacetic acid as internal standard , On μ-BondapakC18 column, with 0.3mol / L tetraethylammonium bromide ethanol solution -20mmol / L dipotassium hydrogen phosphate (volume ratio of 496) as the mobile phase, the detection wavelength of 298nm, quantitative imipenem Determination. Results There was a good linear relationship between imipenem in plasma and plasma concentration of 1 ~ 100μg / ml. The plasma recovery was 98.6% ± 2.25% (n = 6). Intra-day and intra-day plasma RSD were 1.69% ~ 3.76% and 3.82% ~ 6.67%, respectively. The method is simple, rapid, sensitive and accurate; suitable for clinical serum concentration determination.