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目的观察肠胃清协同顺铂(DDP)对HCT116/L-OHP增殖及凋亡的影响。方法用MTT法、流式细胞仪检测肠胃清协同DDP对人结肠癌耐药细胞HCT116/L-OHP增殖及凋亡的影响。结果 HCT116/L-OHP对DDP的耐药指数为2.13倍,48 h DDP作用于HCT116和HCT116/L-OHP的IC50分别为(9.39±0.38),(21.05±1.61)mg·L-1;联用肠胃清后,作用于HCT116/LOHP的IC50降至(13.43±1.22)mg·L-1(P<0.05),逆转指数为1.57倍。联用前后细胞周期分别停滞在G2、S期及G1、S期,细胞凋亡率分别为(19.34±0.38)%,(54±1.77)%(P<0.01)。结论肠胃清能逆转HCT116/L-OHP对DDP的耐药,诱导细胞凋亡,改变周期分布。
Objective To observe the effect of Changwei Decoction combined with DDP on the proliferation and apoptosis of HCT116 / L-OHP. Methods MTT assay and flow cytometry were used to detect the effects of Changwei Decoction and DDP on proliferation and apoptosis of human colon cancer cell line HCT116 / L-OHP. Results The resistance index of HCT116 / L-OHP to DDP was 2.13 times, and the IC50 of HCT116 and HCT116 / L-OHP for 48 h were (9.39 ± 0.38) and (21.05 ± 1.61) mg · L -1, respectively The IC50 of HCT116 / LOHP decreased to (13.43 ± 1.22) mg · L-1 (P <0.05) after rectal clearance, and the reversal index was 1.57-fold. The cell cycle arrest in G2, S and G1, S phases was (19.34 ± 0.38)% and (54 ± 1.77)% (P <0.01), respectively. Conclusion Gengwei can reverse the drug resistance of HCT116 / L-OHP to DDP, induce apoptosis and change the cycle distribution.