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目的:研究马齿苋提取物中6种活性成分在大鼠9种主要组织中的分布情况。方法:采用高效液相色谱法测定口服给予马齿苋提取物后的组织生物样品,以葛根素作为内标物,乙腈-0.1%甲酸水作为流动相梯度洗脱。该研究建立的高效液相色谱法可应用于大鼠心、肝、脾、肺、肾、胃、脑、肠以及肌肉中的咖啡酸、对香豆酸、东莨菪素、阿魏酸、槲皮素和橙皮苷的定量分析。结果:口服给药后,对香豆酸和阿魏酸分布最为广泛,仅在较少组织中检测到咖啡酸、东莨菪素及槲皮素。结论:该实验首次建立了高效液相色谱法同时测定马齿苋提取物中6种活性成分在组织中分布的测定方法。该法简便、快速、重复性好,适用于大鼠口服马齿苋提取物后6种活性成分的体内组织分布研究。
OBJECTIVE: To study the distribution of six active components in nine major tissues of the purslane in the purslane extract. Methods: Tissue samples were obtained by HPLC after oral administration of purslane extract. The mobile phase consisted of puerarin as internal standard and acetonitrile - 0.1% formic acid as mobile phase. The established HPLC can be applied to caffeic acid, p-coumaric acid, scopolamine, ferulic acid and quercetin in rat heart, liver, spleen, lung, kidney, stomach, brain, intestine and muscle Quantitative analysis of peel and hesperidin. RESULTS: Coumadin and ferulic acid were the most widely distributed after oral administration, with caffeic acid, scopolamine and quercetin detected only in less tissues. Conclusion: The method was established for the first time in this experiment to determine the distribution of six active components in the purslane extract by high performance liquid chromatography. The method is simple, rapid and reproducible. It is suitable for the study of tissue distribution of six active ingredients after oral administration of purslane extract in rats.