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目的探讨来源于孕妇羊水和血清的高纯度Glycodelin对E型选择素介导的细胞黏附过程的影响程度。方法以色谱仪分离和纯化孕妇羊水和血清的Glycodelin,测定铬51标记的肝癌细胞系-HepG2细胞在不同来源、不同浓度Glycodelin作用下和重组E型选择素的黏附能力,从而评价Glycodelin对E型选择素介导的细胞黏附过程的影响。结果血清和羊水Glycodelin能有效抑制Hepg2细胞和重组E型选择素之间的黏附,其抑制作用呈浓度依赖关系,其相对抑制力分别为纯四糖结构Sialyl-Lewisx(sLex)的9.4×105和4.0×105倍。结论妊娠相关糖蛋白Glycodelin高度抑制E型选择素介导的细胞黏附。
Objective To investigate the effect of high purity Glycodelin derived from amniotic fluid and serum of pregnant women on E-selectin-mediated cell adhesion. Methods Glycodelin was separated and purified from amniotic fluid and serum of pregnant women by chromatograph, and the adhesion ability of recombinant human hepatocyte carcinoma-HepG2 cells to recombinant E-selectin under different concentrations and concentrations of Glycodelin was evaluated. Selectin-mediated cell adhesion process. Results Glycodelin in serum and amniotic fluid could effectively inhibit the adhesion between Hepg2 cells and recombinant E-selectin in a concentration-dependent manner. The relative inhibitory rates of Glycodelin were 9.4 × 105 and 4.0 × 105, respectively, for the pure tetrasaccharide structure Sialyl-Lewisx 4.0 × 105 times. Conclusion Glycodelin, a glycoprotein of pregnancy, highly inhibits E-selectin-mediated cell adhesion.