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雌性特异血清蛋白存在于成熟雌性大阪鲫鱼血清中，雄鱼则无。注射雌二醇可诱导雄鱼及成熟雌鱼合成这种蛋白质并引起鱼肝细胞粗面内质网增加，糖元颗粒减少。从诱导的大阪鲫鱼血清中分离出电泳纯的雌性特异血清蛋白的分子量为４６６０００±４０００（ｎ＝１０），电泳谱带有３条，用纯的雌性特异血海蛋白制备的兔抗鱼抗血清不与雄鱼血清发生免疫反应，而与正常成熟雌鱼及诱导鱼的血清形成１条免疫沉淀线。免疫细胞化学定位诱导及成熟雌鱼的肝细胞核外细胞质有阳性颗粒，在卵母细胞外围有成团的阳性颗粒分布。
Female specific serum protein is present in the serum of mature female Osaka carassius, but not in male. Injecting estradiol induced the synthesis of this protein by male and mature females and caused an increase in the crude endoplasmic reticulum of fish hepatocytes and a decrease in glycogen granules. The molecular weight of the electrophoresis pure female specific serum protein isolated from the serum of the induced crucian carp was 466000 ± 4000 (n = 10). The electrophoretic profile was 3 and the rabbit anti-fish antisera prepared with pure female specific hemoprotein Not with the male fish serum immune response, but with normal mature female and induced fish serum to form an immunoprecipitation line. Immunocytochemical localization induction and maturation of females hepatocytes extracellular cytoplasm positive particles, in the oocyte peripheral agglomeration of positive particles distribution.