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近年来,一些食品厂家生产含咖啡因的食品越来越多。咖啡因是一种中枢兴奋药,对人体有一定的毒性。目前咖啡因的快速检验方法报道较少,运用薄层色谱的横板技术,对咖啡因的检验进行多次实验,表明方法简便、快速、准确、灵敏度高,适合基层单位大批样品快速检测,介绍如下。咖啡因在酸性水溶液中经氯仿提取,在硅胶GF254薄层板上展式分离,置254nm波长紫外分析仪下观察荧光斑点。将样品与标准比较定性定量。1.仪器:层析槽,薄层板(5×20cm),制板器,血红蛋白吸管,254nm紫外分析仪。2.试剂:硅胶GF254:青岛产。展开剂,氯仿:丙酮:
In recent years, some food manufacturers produce more and more foods that contain caffeine. Caffeine is a central stimulant that has some toxicity to the human body. At present, there are few reports about fast test methods of caffeine. Using thin-layer chromatography horizontal plate technique, many experiments on caffeine testing show that the method is simple, rapid, accurate and sensitive and suitable for rapid detection of a large number of samples in grass-roots units. as follows. Caffeine was extracted by chloroform in acidic aqueous solution and developed on GF254 silica gel plate. Fluorescence spots were observed under 254nm wavelength ultraviolet analyzer. The sample is compared with the standard qualitative and quantitative. 1. Instrument: Chromatography trough, thin plate (5 × 20cm), plate maker, hemoglobin pipette, 254nm UV analyzer. 2. Reagents: Silicone GF254: Qingdao production. Developing agent, chloroform: acetone: