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目的:观察大黄酸对实验性肝纤维化的影响.方法:采用60％的四氯化碳(CCl_4)及5％的乙醇制备肝纤维化动物模型,分别用小剂量、大剂量大黄酸(25 mg/kg,100 mg/kg体重)干预,测定血清丙氨酸氨基转移酶(ALT)、透明质酸(HA)、Ⅲ型前胶(PC-Ⅲ)及肝组织丙二醛(MDA)含量,免疫组化方法观察转化生长因子 β1(TGF-β1)、α平滑肌肌动蛋白(α-SMA)的表达情况,并观察肝组织胶原面积及病理变化.结果:大黄酸组较模型组:(1)血清ALT、HA、PC-Ⅲ水平及肝组织中MDA含量显著降低(P<0.01);(2)肝组织中TGF-β1,α-SMA的表达显著减少(P<0.05或P<0.01);③肝组织胶原面积明显减少,纤维化程度明显改善(P<0.05或P<0.01).结论:大黄酸具有保肝作用和抑制肝纤维化作用,其作用机制可能与其抗炎、抗氧化作用及抑制HSC活化、抑制TGF-β1作用有关.
Objective: To observe the effect of rhein on experimental hepatic fibrosis.Methods: Liver fibrosis was induced by 60% carbon tetrachloride (CCl 4) and 5% ethanol, and then treated with low dose and high dose rhein (25 (ALT), hyaluronic acid (HA), type Ⅲ procollagen (PC-Ⅲ), and malondialdehyde (MDA) The expression of transforming growth factor-β1 (TGF-β1) and α-smooth muscle actin (α-SMA) was observed by immunohistochemistry and the collagen area and pathological changes were observed.Results: Compared with the model group 1) The levels of serum ALT, HA, PC-Ⅲ and the content of MDA in liver tissue were significantly decreased (P <0.01). (2) The expression of TGF- ); ③The area of collagen in liver tissue was significantly reduced and the degree of fibrosis was significantly improved (P <0.05 or P <0.01) .Conclusion: Rhein has hepatoprotective effect and hepatic fibrosis inhibitory effect, its mechanism may be related to its anti-inflammatory and anti-oxidation Role and inhibition of HSC activation, inhibition of the role of TGF-β1.