论文部分内容阅读
目的探讨P21蛋白和增殖细胞核抗原(proliferating cell nuclearantigen,PCNA)在子宫内膜癌中的表达及意义。方法应用免疫组织化学SP法检测正常子宫内膜组织20例(对照组)、子宫内膜非典型增生组织9例(非典型增生组)和子宫内膜腺癌组织57例(子宫内膜癌组)中P21蛋白和PCNA的表达情况。结果对照组P21蛋白阳性表达率明显高于非典型增生组与子宫内膜癌组(P<0.05),子宫内膜癌组PCNA高增殖率明显高于对照组与非典型增生组(P<0.05);P21蛋白阳性表达率及PCNA高增殖率在子宫内膜组织的手术-病理分期、分化程度及淋巴结转移方面差异有统计学意义(P<0.05);子宫内膜癌组PCNA高增殖率P21表达阳性者为37%,P21表达阴性者为80%,差异有统计学意义(P<0.05);子宫内膜癌组织P21蛋白阳性表达率与PCNA高增殖率呈负相关(r=-0.414,P=0.002)。结论 P21蛋白和PCNA异常表达可能在子宫内膜癌的发生、发展中起重要作用。
Objective To investigate the expression of P21 protein and proliferating cell nuclear antigen (PCNA) in endometrial carcinoma and its significance. Methods 20 cases of normal endometrium (control group), 9 cases of endometrial dysplasia (atypical hyperplasia) and 57 cases of endometrial adenocarcinoma (endometrial carcinoma ) P21 protein and PCNA expression. Results The positive expression rate of P21 protein in control group was significantly higher than that in atypical hyperplasia group and endometrial carcinoma group (P <0.05). The high proliferative rate of PCNA in endometrial carcinoma group was significantly higher than that in control group and atypical hyperplasia group (P <0.05) ). The positive expression rate of P21 protein and high proliferative rate of PCNA in endometrial tissue were significantly different in operation-pathological stage, differentiation degree and lymph node metastasis (P <0.05). The proliferative rate of PCNA in endometrial carcinoma group was significantly higher than that in P21 (P <0.05). The positive rate of P21 protein in endometrial carcinoma was negatively correlated with the high proliferative rate of PCNA (r = -0.414, P < P = 0.002). Conclusion The abnormal expression of P21 protein and PCNA may play an important role in the occurrence and development of endometrial carcinoma.