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目的:建立甘肃南部地区泽漆药材的高效液相指纹图谱,为泽漆的质量控制提供有效方法。方法:采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,流速为1.0ml·min-1,检测波长为360 nm,柱温为30℃;采用“中药指纹图谱相似度评价系统(2004 A版)”确定共有峰,进行模式分析及相似度计算,并根据共有峰的相对峰面积对30批样品使用SPSS 17.0进行聚类分析。结果:指认了指纹图谱中11个共有峰,各批次样品成分类型基本一致,但相似度不高,药材成分特征与其产地地理位置间无明显关联。结论:首次建立了泽漆药材的高效液相指纹图谱,该方法简便准确,灵敏度高,重复性好,可作为泽漆的质量控制方法。
Objective: To establish a HPLC fingerprint of Radix Laccase in the southern part of Gansu, and to provide an effective method for the quality control of Radix Lacquer. METHODS: The mobile phase was eluted with an Agilent Eclipse XDB-C18 column (250 mm × 4.6 mm, 5 μm) using acetonitrile-0.1% phosphoric acid as mobile phase with a flow rate of 1.0 ml · min-1 and a detection wavelength of 360 nm. The column temperature was 30 ℃. The similarity of fingerprint was evaluated using the Chinese Medicinal Fingerprint Similarity Evaluation System (Version 2004A), and the pattern analysis and the similarity calculation were performed. Based on the relative peak area of the common peaks, SPSS 17.0 Cluster analysis. Results: The 11 common peaks in fingerprints were identified. The sample types in each batch were basically the same, but the similarity was not high. There was no significant correlation between the components of medicinal materials and the geographic location of their origin. Conclusion: The HPLC fingerprinting of Radix Laccase is established for the first time. The method is simple, accurate, sensitive and reproducible. It can be used as the quality control method of Radix Lacquer.