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目的:探讨猴鼻腔一致性切片制作方法,用于临床前鼻腔给药、吸入途径或其他途径给药药物的猴鼻腔组织病理学检查。方法:通过对食蟹猴鼻腔的解剖、固定、脱钙后,着重探讨了用于鼻腔给药药物及吸入毒性研究的猴鼻腔毒性病理检查一致性鼻腔标本4个切面的取材方法及参考的大体解剖标志,第1个切面在第1前磨牙前;第2个切面在第1、第2前磨牙之间;第3个切面在第1磨牙后,包括鼻腔旁边的上颌窦;第4个切面在鼻咽部软腭的前端和后端中间的位置。同时探讨了猴鼻腔组织HE染色切片制片中的其它注意事项。结果:制作出符合毒性病理学检查的猴鼻腔4个切面的HE染色切片,并清楚地显示了不同鼻粘膜上皮,包括鳞状上皮、移行上皮、嗅上皮和呼吸上皮。结论:本文描述了猴鼻腔毒性病理学检查的标准制片方法,运用本制片方法可以很好地制作出符合猴鼻腔毒性病理学镜检要求的一致性切片,用于临床前鼻腔给药药物、吸入途径或其它给药途径给药研究的猴鼻腔组织病理学检查。
OBJECTIVE: To investigate the method for producing nasopharyngeal consistent sections of monkeys for use in nasal histopathological examination of pre-clinical nasal administration, inhalation route or other routes of administration. Methods: After the anatomy, fixation and decalcification of the nasal cavity of cynomolgus monkeys, the four nasopharyngeal specimens of nasopharyngeal toxicity and pathogenicity of nasopharyngeal drug and inhalation toxicity study were mainly discussed. Anatomical landmarks, the first section in front of the first premolar; the second section between the first and second premolar; the third section after the first molar, including the maxillary sinus next to the nasal cavity; the fourth section Position in the middle of the front and back of the nasopharynx soft palate. At the same time, other considerations of monkey nasal tissue HE staining were also discussed. RESULTS: HE-stained sections of four sections of the monkey nasal cavity that were toxicologically pathologically examined were made and clearly showed different nasal mucosal epithelium including squamous epithelium, migratory epithelium, olfactory epithelium and respiratory epithelium. Conclusion: This article describes a standard nasopharyngeal nasopharyngeal pathological examination of the production method, the use of this production method can be well produced in line with the monkey nasal toxicology pathology microscopy consistent sections for pre-nasal administration of drugs , Nasal histopathological examination of inhalation routes or other routes of administration for administration.