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目的探讨安多霖对微波辐照致大鼠脑神经元尼氏体损伤的预防作用。方法 40只二级雄性Wistar大鼠随机分为5组:正常对照组、辐照对照组、0.75 g/(kg.d)组、1.5 g/(kg.d)组及3 g/(kg.d)组。给药组每日1次灌胃给予安多霖溶液,连续给药2 w。给药结束后采用30 mW/cm2微波辐照大鼠,辐射时间为15 min。于停药(辐照)后6 h、7 d和14 d取大鼠海马和大脑皮层,采用甲苯胺蓝染色和图像分析技术,定量研究神经元尼氏体含量的变化。结果微波辐照后6 h,大鼠海马和皮层神经元尼氏体含量明显减少(P<0.01);辐照后7 d,尼氏体含量基本恢复。0.75 g/(kg.d)安多霖预防组上述变化与辐照对照组相似。而1.5 g/(kg.d)和3 g/(kg.d)预防组在照后尼氏体含量无明显改变。结论 30 mW/cm2微波辐照可引起大鼠海马和皮层神经元尼氏体含量减少;1.5 g/(kg.d)和3 g/(kg.d)安多霖对微波辐照致大鼠脑神经元尼氏体含量减少有一定预防作用;其中1.5 g/(kg.d)安多霖为预防微波辐照致大鼠脑神经元尼氏体含量减少的有效剂量。
Objective To investigate the preventive effect of Andolin on the Nissl body injury induced by microwave in rat brain neurons. Methods Forty male Wistar rats were randomly divided into five groups: normal control group, irradiation control group, 0.75 g / (kg.d) group, 1.5 g / (kg.d) group and 3 g / (kg) d) Group. Administered group once daily intragastric administration of Andorium solution, continuous administration of 2 w. Rats were irradiated with 30 mW / cm2 microwave after irradiation for 15 min. The hippocampus and cerebral cortex of rats were taken at 6 h, 7 d and 14 d after withdrawal (irradiation). Toluidine blue staining and image analysis were used to quantitatively study the change of Nissl body content in neurons. Results At 6 h after microwave irradiation, the content of Nissl body in hippocampus and cortex of rats significantly decreased (P <0.01). At 7 d after irradiation, the content of Nissl body recovered. 0.75 g / (kg.d) Andolimin prevention group The above changes are similar to the irradiated control group. However, there was no significant change in the content of Nissl after 1.5 g / (kg · d) and 3 g / (kg · d) prophylaxis groups. Conclusion Microwave irradiation of 30 mW / cm2 can reduce the content of Nissl body in hippocampus and cortical neurons of rats. Under the action of 1.5 g / (kg · d) and 3 g / (kg · d) Neurons reduce the content of Nissl had a preventive effect; Of which 1.5 g / (kg.d) Andorol Lin to prevent microwave radiation-induced neuronal Nissl reduce the effective dose of the body.