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目的:建立家兔血液及关节微透析液中青藤碱的高效液相色谱-质谱联用测定方法,并应用于青藤碱在家兔体内的整体药动学及局部药动学的研究。方法:采用Hypersil GOLD C18柱(150mm×2.1mm,3μm),流动相为甲醇-0.1%甲酸(30∶70),流速0.2mL·min-1,质谱为电喷雾离子源,选择反应离子监测,质荷比为239和181,采用外标法,微透析样品直接进样分析。结果:在0.336~172μg·L-1范围内呈线性关系良好,r=0.9972,定量下限为0.336μg·L-1,低、中、高浓度质控样品的日内及日间精密度符合要求。结论:本方法灵敏度高,操作简便,能满足体内微透析样品中低浓度的测定,可用于青藤碱药物动力学特性的研究
OBJECTIVE: To establish a method for the determination of sinomenine in blood and joint microdialysis in rabbits by HPLC-MS method and to study the pharmacokinetics and pharmacokinetics of sinomenine in rabbits. METHODS: Hypersil GOLD C18 column (150 mm × 2.1 mm, 3 μm) was used. The mobile phase consisted of methanol-0.1% formic acid (30:70) and the flow rate was 0.2 mL · min-1. The mass spectrometry was electrospray ionization source. Mass-to-charge ratio of 239 and 181, using external standard method, direct dialysis sample analysis. Results: The linearity was good in the range of 0.336 ~ 172μg · L-1, r = 0.9972, and the lower limit of quantification was 0.336μg · L-1. The precision of intra-day and inter-day precision of low, middle and high quality control samples was satisfactory. Conclusion: The method is sensitive and easy to operate and can meet the requirement of low concentration in microdialysis samples in vivo. It can be used to study the pharmacokinetics of sinomenine