论文部分内容阅读
为了解广西近年确诊的HIV-1感染者流行病学及HIV-1亚型分布情况,分别在桂林、来宾、钦州采取样本100例、102例和103例,收集流行病学资料、采集抗凝全血、提取病毒RNA、用RT-PCR进行gag基因扩增并对产物进行测序和序列分析。结果显示:在305例样本中,男女性别比1.99∶1;青壮年年龄组患者占56.72%,>50岁年龄组患者构成比例为44.62%;职业为农民的患者比例为50.82%;初中及以下文化水平患者占76.39%;经异性性接触途径传播构成比为95.74%。最终成功扩增得到155份样本的gag基因序列,共发现4种亚型:CRF01-AE亚型124例、CRF07-BC亚型12例、CRF08-BC亚型14例、B亚型5例。
In order to understand the epidemiology and HIV-1 subtypes of HIV-1 infected patients diagnosed in recent years in Guangxi, 100 samples, 102 samples and 103 samples were collected from Guilin, Laibin and Qinzhou respectively to collect epidemiological data and collect anticoagulants Whole blood, viral RNA was extracted, gag gene amplification was performed by RT-PCR and the product was sequenced and sequenced. The results showed that the male-female ratio was 1.99: 1 in 305 samples, 56.72% in young adults, 44.62% in patients aged> 50 and 50.82% The level of education was 76.39%, and the ratio of heterosexual exposure was 95.74%. Finally, we successfully amplified 155 samples of gag gene sequences, found a total of four subtypes: CRF01-AE subtype 124 cases, CRF07-BC subtype 12 cases, CRF08-BC subtype 14 cases, B subtype 5 cases.