An optimization model for the regional comprehensive freight hub location problem was built up from the viewpoint of the minimum cost of freight system. The construction method for regional comprehensive intermodal freight network was put forward, and three constraints of the reasonable and feasible path of the intermodal transport, i. e. , reasonable sequence, reasonable freight distance, and reasonable conversion times, were proposed according to the general concepts of the intermodal freight. Then the method for solving the model was given based on the super network and genetic algorithm. Finally, the model and the solution were verified by case study.