炼苗是生物组培技术中较为关键的一环,它直接关系到组培苗能否尽快地应用到大田生产中去。试管苗移栽过程较为复杂,盲目移栽或稍有不慎,就会造成大批组培苗死亡。因此,研究组培苗与炼苗室中小气候环境诸要素之间的相互关系尤为重要。笔者试图从炼苗室小气候入手来探讨炼苗室中组培苗的锻炼与驯化技术。 炼苗的环境条件 试管苗比较娇嫩,它从瓶子中移到炼苗室中,首要一条,温度要尽可能保持同培养室的一致,湿度要尽可能增大,否则易导致幼苗停止生长,甚至死亡。温湿度的调节是炼苗的核心问题。 Hardening is the biological tissue culture more critical part of the technology, which is directly related to tissue culture seedlings can be applied to field production as soon as possible. Test tube seedling transplanting process is more complicated, blind transplanting or a little careless, it will result in a large number of tissue culture seedlings die. Therefore, it is particularly important to study the relationship between the tissue culture seedlings and the elements of microclimate environment in the practice room. The author tries to discuss the cultivation and acclimation techniques of tissue culture seedlings in the practice room from the microclimate of the practice room. The environmental conditions of hardened potting plantlets more delicate, it moved from the bottle into the practice room, the first one, the temperature should be as consistent as possible with the training room, humidity should be increased as much as possible, or easily lead to seedlings stop growing, or even death. The regulation of temperature and humidity is the core issue of seedling raising.