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本研究以pBI121为植物表达载体，在已构建的无融合生殖基因表达载体pBI121-MhSERK1和pBI121-MhdSER K 1的基础上，利用根癌农杆菌介导法，将苹果属无融合生殖相关基因SERK1转入受体植株烟草中。研究结果表明：烟草叶片浸染后，经共培养和选择培养形成再生芽，分别获得7株pBI121-MhSER K 1和6株pBI121-MhdSER K 1转烟草抗性植株，PCR检测证明已成功获得MhSER K 1和MhdSER K 1烟草转化株系。本研究可为下一步无融合生殖相关基因功能验证研究奠定基础。“,”In this study, based on the constructed expression vectors of pBI121-MhSERK1 and pBI121-MhdSERK1, we transferred an apomixis related-gene in Malus, SERK1, to tobacco plants with pBI121 as expression vectors by A grobacterium tumefaciens-mediated transformation. The results showed that the transgenic plants of 7 individuals with pBI121-MhSER K 1 and 6 individuals with pBI121-MhdSER K 1 were obtained after co-culture and selective culture the tobacco leaves, and PCR detection demonstrated that MhSER K 1 and MhdSER K 1 were transferred into tobacco successfully. The study lay a foundation of apomixis gene proving genes function.