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目的 探讨神经生长因子 (NGF)和丹参对新生大鼠缺氧缺血性脑病 (HIE)的防治作用。方法 选用 7日龄新生大鼠 ,在制成HIE模型前后给予NGF和丹参腹腔注射 ,HIE后分期处死大鼠分别测定谷氨酸 (Glu) ,天门冬氨硫 (Asp) ,一氧化氮合酶 (NOS)和做病理检查。 结果 HIE组 (30min)Glu ,Asp和 (48h)NOS较正常对照组明显升高。Glu :(887.0± 145 .2 ) μg/g ,vs.(776 .5± 10 3.0 ) μg/g ,(P <0 .0 5 ) ;Asp :(2 78.6± 48.2 ) μg/g ,vs.(2 2 4.8± 2 7.0 ) μg/g ,(P <0 .0 1) ;NOS :(3.34 6± 0 .745 )U/ml,vs.(2 .195± 0 .6 15 )U/ml,(P <0 .0 1)。NGF和丹参可以抑制HIE后的Glu ,Asp和NOS升高 ,并可减轻脑组织病理改变。Glu :(6 94.9± 83.9) μg/g ,vs .(887.0± 145 .2 ) μg/g ,(P <0 .0 1) ;Asp :(2 0 6 .1± 2 5 .4) μg/g ,vs.(2 78.6± 48.2 ) μg/g ,(P <0 .0 1) ;NOS :(2 .0 41± 0 .35 7)U/ml,vs.(3.34 6± 0 .745 )U/ml,(P <0 .0 1)。结论 NGF和丹参对HIE有防治作用。
Objective To investigate the preventive and therapeutic effects of nerve growth factor (NGF) and radix salviae miltiorrhizae on hypoxic-ischemic encephalopathy (HIE) in neonatal rats. Methods Neonatal 7-day-old neonatal rats were injected intraperitoneally with NGF and Salvia miltiorrhiza before and after the HIE model. Rats were sacrificed at the end of HIE, and glutamate (Glu), aspartate (Asp), nitric oxide synthase (NOS) and pathological examination. Results HIE group (30min) Glu, Asp and (48h) NOS than the normal control group was significantly higher. Glu: (887.0 ± 145.2) μg / g, vs. (776.5 ± 10 3.0) μg / g, (P <0.05); Asp: (786 ± 48.2) μg / g, (2 2 4.8 ± 2 7.0) μg / g, (P <0.01); NOS: (3.34 6 ± 0. 745) U / ml vs , (P <0. 01). NGF and Salvia miltiorrhiza could inhibit the increase of Glu, Asp and NOS after HIE and alleviate the pathological changes of brain tissue. Glu: (694.9 ± 83.9) μg / g vs (887.0 ± 145.2) μg / g, P <0.01; Asp: (206 ± 2.54) μg / (2 78.6 ± 48.2) μg / g, (P <0.01); NOS: (2.041 ± 0.357) U / ml vs.334 6 ± 0.745 U / ml, (P <0. 01). Conclusion NGF and Salvia have a preventive and therapeutic effect on HIE.