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目的探讨表皮生长因子受体1(ErbB1)与ErbB3基因在新疆经典型Kaposi肉瘤(kaposi′s sarcoma,KS)发病过程中的表达情况。方法分别采用实时荧光定量PCR和免疫组化技术,对17例新疆经典型KS患者的肿瘤组织和瘤旁正常组织中ErbB1与ErbB3基因的表达进行检测。结果 ErbB1,ErbB3基因在KS肿瘤组织中mRNA的表达均低于瘤旁正常组织中的表达,差异有统计学意义(P<0.05);在KS与KS瘤旁正常组织中,ErbB1蛋白阳性表达率分别为11.8%和94.1%;ErbB3蛋白阳性表达率分别为17.6%和94.1%,ErbB1,ErbB3蛋白在KS中的表达明显低于在KS瘤旁正常组织中的表达,差异均有统计学意义(P<0.005)。结论 ErbB1和ErbB3基因在KS组织中呈现低度表达或不表达,提示其在新疆经典型KS肿瘤细胞增殖中可能作用有限。
Objective To investigate the expression of ErbB1 and ErbB3 in the pathogenesis of Kaposi’s sarcoma (KS) in Xinjiang. Methods The mRNA expressions of ErbB1 and ErbB3 in tumor tissue and adjacent normal tissues of 17 patients with classic KS in Xinjiang were detected by real-time fluorescence quantitative PCR and immunohistochemistry respectively. Results The mRNA expression of ErbB1 and ErbB3 in KS tumor tissues was lower than that in the adjacent normal tissues (P <0.05). The positive expression rates of ErbB1 protein in KS and KS normal tissues Respectively, 11.8% and 94.1% respectively. The positive rates of ErbB3 protein were 17.6% and 94.1%, respectively. The expression of ErbB1 and ErbB3 protein in KS was significantly lower than that in normal tissue adjacent to KS (the difference was statistically significant P <0.005). Conclusions ErbB1 and ErbB3 genes are low or not expressed in KS tissues, suggesting that ErbB1 and ErbB3 genes may play a limited role in the proliferation of classic KS tumor cells in Xinjiang.