目的从小牛肝脏中分离纯化具有抑制肝癌细胞活性的多肽类物质,并分析其性质。方法将小牛肝脏细胞的提取液经超滤、透析、加热、乙腈沉淀、离子交换层析等步骤分离、纯化得到几种蛋白并在体外用HepG2细胞检测其抗肿瘤活性。结果获得了2种对肝癌细胞有显著抑制效应的蛋白,其分子量分别为10.9KD和11.9KD。结论发现两种对热很稳定的非精氨酸酶类新的肝源性细胞生长抑制因子,有望发展成为新的治疗肝癌的药物。 Objective To isolate and purify the polypeptides that inhibit the activity of hepatoma cells from calf liver and analyze their properties. Methods The calf liver cell extracts were isolated by ultrafiltration, dialysis, heating, acetonitrile precipitation and ion exchange chromatography. Several proteins were purified and their anti-tumor activities were detected by HepG2 cells in vitro. Results Two kinds of proteins with significant inhibitory effect on hepatocarcinoma cells were obtained, with molecular weights of 10.9KD and 11.9KD, respectively. Conclusions Two novel non-arginase-like hepatocyte growth inhibitory factors, which are very stable to heat, have been found to be promising drugs for the treatment of liver cancer.