论文部分内容阅读
目的研究肝泡状棘球蚴感染对大鼠免疫学状态及移植心存活的影响。方法建立SD大鼠到W istar大鼠的颈部异位心脏移植模型。对照组以未接种泡状棘球蚴的W istar大鼠为受者;实验组以感染泡状棘球蚴的W istar大鼠为受者。术后观察移植心的存活时间、组织病理学变化、心肌组织内T淋巴细胞和嗜酸粒细胞的浸润情况,测定血清内白细胞介素4(IL-4)和γ干扰素(IFN-γ)水平。结果实验组移植心的存活时间与对照组相比,差异有统计学意义(P<0.05),组织病理学分级及心肌组织中CD4+T淋巴细胞数与对照组相比,差异无统计学意义,而CD8+T淋巴细胞数、嗜酸粒细胞数与对照组相比,差异均有统计学意义。发生排斥反应时,实验组血清内IL-4水平高于对照组,而IFN-γ水平低于对照组。结论泡状棘球蚴感染造成TH1/TH2向TH2类细胞因子偏移,有利于移植物的存活,嗜酸粒细胞浸润可能是移植心发生排斥反应的原因之一。
Objective To study the effect of hepatic cirrhosis infection on immunological status and graft survival in rats. Methods The cervical heterotopic heart transplantation model from SD rats to Wistar rats was established. In the control group, Wistar rats that were not inoculated with Echinococcus granulosus were used as recipients. In the experimental group, Wistar rats infected with Echinococcus granulosus were used as recipients. Survival time, histopathological changes, infiltration of T lymphocytes and eosinophils in myocardium were observed after operation. Interleukin-4 (IL-4) and interferon-γ (IFN-γ) Level. Results Compared with the control group, the survival time of heart transplantation in the experimental group was statistically significant (P <0.05). The histopathological grade and the number of CD4 + T lymphocytes in myocardium were not significantly different from those in the control group , While the number of CD8 + T lymphocytes, eosinophils and the control group, the differences were statistically significant. When rejection occurred, the serum level of IL-4 in the experimental group was higher than that in the control group, while the level of IFN-γ was lower than that in the control group. Conclusions The infection of Cystic echinococcus causes TH1 / TH2 to migrate to TH2 cytokines, which is beneficial to graft survival. Eosinophil infiltration may be one of the reasons for graft rejection.