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目的:探讨利用细胞块检测肾小球疾病患者尿中足细胞和巨噬细胞及其与肾脏病理及功能损害的关系,试图寻找一种反映肾损害的非创伤性指标。方法:利用免疫组化测定患者尿细胞块中足细胞和巨噬细胞数,并用HE和免疫组织化学双染技术观察肾脏活检病理切片,用化学法测定尿蛋白、血肌酐和尿肌酐。结果:肾小球疾病患者尿细胞块中足细胞和巨噬细胞数目比正常对照明显增多(P<0.01);尿细胞块中足细胞和巨噬细胞数目与蛋白尿程度、肾组织损害指数及肾功能损害明显相关(P<0.01)。结论:肾小球疾病患者尿细胞块中足细胞和巨噬细胞明显增多,并且与蛋白尿程度、肾组织损害指数及肾功能损害明显相关,提示其作为一种监测肾小球疾病肾损害的非创伤性指标的可能。
OBJECTIVE: To explore the relationship between urinary podocytes and macrophages in renal glomerulus patients and their relationship with renal pathological and functional impairment, and to find a noninvasive indicator of renal damage. Methods: Immunohistochemistry was used to determine the number of podocytes and macrophages in urinary cell mass. The pathological sections of renal biopsy were observed by HE and immunohistochemical staining. Urine protein, serum creatinine and urinary creatinine were measured by chemical method. Results: The numbers of podocytes and macrophages in urinary cell masses were significantly increased in patients with glomerular diseases (P <0.01). The number of podocytes and macrophages, the level of proteinuria, the index of renal damage, Renal dysfunction was significantly correlated (P <0.01). CONCLUSIONS: Glomerular disease patients have significantly increased numbers of podocytes and macrophages in the urine cell mass, and are significantly associated with the degree of proteinuria, renal damage index and renal dysfunction, suggesting that as a marker of renal damage in glomerular diseases The possibility of non-invasive indicators.