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目的研究细胞间黏附分子-1(ICAM-1)在粉防己碱(TET)对抗移植心脏慢性排斥反应中的作用。方法Wistar-Wistar和SD-Wistar大鼠间的颈部心脏移植分别作为同系和异系对照。TET治疗组(SD-Wistar)大鼠每天另给予50mg/kgTET灌胃。异系对照和TET治疗组大鼠每日给予环孢素A灌胃(1.5mg/kg),同系对照组不用任何药物治疗。12周后取移植心脏做组织学检查,免疫组织化学法检测组织中ICAM-1表达,以图像分析仪计算阳性率。结果异系对照动脉管壁占血管直径的平均比值为0.64±0.20,明显高于同系对照(0.23±0.07,P<0.01)和TET治疗组(0.33±0.11,P<0.05);ICAM-1在同系对照低度表达,异系对照的表达明显增强,TET治疗组的ICAM-1表达则明显减少。结论ICAM-1在慢性排斥的发生中具有重要作用,抑制ICAM-1表达可能是TET抗慢性排斥的作用机制之一。
Objective To study the role of intercellular adhesion molecule-1 (ICAM-1) in the prevention of chronic rejection of transplanted heart with tetrandrine (TET). Methods Cervical heart transplantation between Wistar-Wistar and SD-Wistar rats served as syngeneic and heterologous controls, respectively. SD-Wistar rats were intragastrically administered with 50 mg/kg TET daily. The rats in the heterologous control and TET treatment group were given cyclosporine A daily (1.5 mg/kg), and the same control group was not treated with any drug. After 12 weeks, the transplanted heart was taken for histological examination. The expression of ICAM-1 in the tissue was detected by immunohistochemistry and the positive rate was calculated with an image analyzer. Results The mean ratio of the arterial wall to the vascular diameter of the heterologous control group was 0.64±0.20, which was significantly higher than that of the syngeneic control (0.23±0.07, P<0.01) and TET treatment group (0.33±0.11, P<0.05); ICAM-1 was The low-level expression of syngeneic control, the expression of heterologous control was significantly enhanced, and the expression of ICAM-1 in TET-treated group was significantly reduced. Conclusion ICAM-1 plays an important role in the development of chronic rejection. Inhibiting the expression of ICAM-1 may be one of the mechanisms of TET anti-chronic rejection.