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为探讨微波照射对人外周血NK细胞活性的影响 ,取成人静脉血 ,分离得NK细胞后 ,用 2 45 0MHz微波进行照射 ,用改良的乳酸脱氢酶 (LDH)释放法测定NK细胞活性 (NaturalKillerCellActivity ,NKCA)。结果显示 :10mA、 2 0mA、 40mA三种强度照射 15分钟后 ,NKCA随照射强度的增大而增强 ;强度为 2 0mA分别照射 10分钟、 15分钟、 2 0分钟和 2 5分钟后 ,随照射时间延长 ,NKCA呈不同程度的增强反应 ,以 2 0分钟组增强最显著 ,10分钟和 2 5分钟组次之 ,15分钟组增强不显著。说明人外周血NK细胞经适当强度和时间的微波照射后 ,活性可有不同程度的增强
To investigate the effect of microwave irradiation on the activity of NK cells in human peripheral blood, NK cells were isolated from adult human venous blood and irradiated with 2,400 MHz microwaves. The activity of NK cells was measured by modified lactate dehydrogenase (LDH) release assay NaturalKiller CellActivity, NKCA). The results showed that NKCA tended to increase with the increase of irradiation intensity at 10mA, 20mA and 40mA irradiation intensity for 15 minutes. The intensity of 20mA irradiation for 10 minutes, 15 minutes, 20 minutes and 25 minutes, respectively, With prolongation of time, NKCA increased to some extent, with the most significant increase in 20 minutes, followed by 10 minutes and 25 minutes, and no significant increase in 15 minutes. This shows that human peripheral blood NK cells by appropriate intensity and time of microwave irradiation, the activity may have different levels of enhancement