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为分离得到高温高效降解菌,促进堆肥腐熟、提高堆肥品质,采用稀释平板法和水解试验初筛以及酶活性复筛的方法,从好氧堆肥样品中分离得到一株可同时高效降解纤维素、蛋白质和淀粉的高温细菌HN-5,其纤维素酶活性、蛋白酶活性和α-淀粉酶活性分别为7.308、13.296和76.136U/mL。通过对菌株的形态特征观察、部分生理生化实验和16SrDNA基因鉴定,初步确定其为Geobacillus属的菌株。菌株最适生长温度为60℃;最适生长pH为6.0;在NaCl质量浓度为10mg/mL、装液量为10%(体积分数)时,菌株生长量最大。将HN-5接种至鸡粪+锯末堆肥中进行堆肥效果验证,结果表明接种HN-5可以有效地促进堆肥升温,延长高温期持续时间。
In order to separate the high temperature and efficient degrading bacteria, promote compost composting and improve the quality of compost, the method of dilute plate method and hydrolysis test screening and enzyme activity screening was used to separate and purify one aerobic composting sample which can effectively degrade cellulose, Protein and starch of high temperature bacteria HN-5, the cellulase activity, protease activity and α-amylase activity were 7.308,13.296 and 76.136U / mL. Through the observation of morphological characteristics of strains, some physiological and biochemical experiments and 16SrDNA gene identification, initially identified as Geobacillus genus strains. The optimum growth temperature of the strain was 60 ℃. The optimum growth pH was 6.0. When the concentration of NaCl was 10 mg / mL and the volume of liquid was 10%, the strain grew most. HN-5 was inoculated into chicken manure + sawdust composting to verify the composting effect. The results showed that inoculation of HN-5 could effectively promote the compost warming and prolong the duration of high temperature.