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从 2 0 0 1年 10月开始 ,中国疾病预防控制中心病毒病预防控制所国家脊髓灰质炎 (脊灰 )实验室 ,增加使用酶联免疫吸附试验 (ELISA)对从急性弛缓性麻痹 (AFP)病例粪便标本中分离到的脊灰病毒进行型内鉴别。 2 0 0 2年6 0 7株单型脊灰病毒的ELISA试验结果是 :5 73株为疫苗相似株 (SL) ,3株为非疫苗相似株 (NSL) ,2 9株为双反应株 (DRV) ,2株为阴性反应株 (NRV)。将ELISA试验结果与逆转录 聚合酶链反应 限制性内切酶片段长度多态性分析 (RT PCR RFLP)结果进行比较 ,发现仅 2株ELISA试验结果为DRV的脊灰病毒 ,PCR结果为变异株。同样 ,在 33株PCR结果为变异株的脊灰病毒中 ,仅有 2株ELISA试验结果为DRV。由于两种方法的原理不同 ,它们得出的结论并无很高的一致性。将ELISA试验结果为NSL和DRV、PCR结果为变异株的毒株进行了脊灰病毒全VP1区核苷酸序列分析 ,在ELISA试验结果为DRV和NSL的脊灰病毒的全VP1区氨基酸序列分析结果中 ,仅有Ⅰ型脊灰病毒的氨基酸变异位点在已知的SabinⅠ型抗原决定簇位点 1(site 1)内 ,Ⅱ型和Ⅲ型的变异位点均不在其中 ,这一矛盾的结果说明 ,可能在VP1区以外其它编码区的抗原决定簇起到主要作用 ,或者试剂盒本身的检测系统存在某些需要改进的地方。在ELISA试验过程中 ,发现Ⅲ型试剂
Beginning from October 2001, the National Poliovirus (Polio) Laboratory of the Virus Disease Prevention and Control Center of the Chinese Center for Disease Control and Prevention has increased the use of enzyme linked immunosorbent assay (ELISA) to detect acute flaccid paralysis (AFP) Poliovirus isolated from the stool samples were typed in-vivo. The ELISA results of 607 single-poliovirus strains in 2002 showed that 5 73 strains were vaccine-like strains (SL), 3 were non-vaccine-like strains (NSL) and 29 were double-reaction strains DRV) and two strains were negative (NRV). The results of ELISA and RT PCR RFLP were compared and found that only two ELISA test results for the DRV of poliovirus, PCR results for the mutant . Similarly, out of the 33 polioviruses mutated, only two of the ELISA tests showed DRV. Due to the different principles of the two methods, they conclude that there is not a high degree of agreement. The results of ELISA test for NSL and DRV, PCR results for the mutant strain of poliovirus full VP1 nucleotide sequence analysis of the ELISA test results for the DRV and NSL poliovirus full VP1 region amino acid sequence analysis As a result, only the amino acid variation site of type I poliovirus was within known site 1 of Sabin type I epitopes, and neither type II nor type III variant sites were present The results indicate that it is possible that the epitopes in other coding regions outside of the VP1 region play a major role or that there is some room for improvement in the detection system of the kit itself. During ELISA testing, type III agents were found