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目的:探讨stTRAIL-MSC靶向促进肝癌大鼠RFA过渡区残癌细胞凋亡的作用。方法:对150只雄性SD大鼠,采用N1S1肿瘤细胞建立肝荷瘤大鼠模型,并随机将其分为stSTRAL-MSC组、MSC组、Vector-MSC组、培养液组以及空白对照组,每组30只,在接受治疗前1周给以注射相应的制剂,在接受RFA治疗后12 h、24 h、48 h以及1周时每组取6只处死并进行相关检查(空白组除外),同时比较空白组与RFA组其生存时间。结果:stTRAIL-MSC在接受RFA治疗后其肿瘤体积变化、过渡区肿瘤细胞凋亡指数、大鼠肿瘤毁损体积、过渡区肿瘤细胞(ki-67染色)增殖指数等比较明显优于其他几组,而且在对caspase-3、caspase-8、Bcl-2水平检测时发现,stTRAIL-MSC其caspase-、3caspase-8水平明显高于其他四组,而Bcl-2水平低于其他四组,对接受RFA以及空白对照组大鼠的生存情况分析发现,采用RFA治疗的大鼠其生存时间明显高于空白对照组。结论:stTRAIL-MSC靶向通过提高cas-pase蛋白酶级联反应继而达到促进肝癌大鼠RFA过渡区残癌细胞凋亡。
Objective: To investigate the effect of stTRAIL-MSC targeting on the apoptosis of tumor cells in the RFA transitional region of hepatocarcinoma rats. Methods: 150 male Sprague-Dawley rats were used to establish the model of hepatic tumor-bearing rats with N1S1 tumor cells and randomly divided into stSTRAL-MSC group, MSC group, Vector-MSC group, culture medium group and blank control group (N = 30). The rats were injected with the corresponding preparations one week prior to the treatment. Six rabbits in each group were sacrificed at 12 h, 24 h, 48 h and 1 week after RFA treatment (except for the blank group) Meanwhile, the survival time of blank group and RFA group was compared. Results: The tumor volume of stTRAIL-MSC after RFA treatment was significantly higher than that of other groups. The apoptosis index of tumor cells in transitional area, the volume of tumor destruction in rat, the proliferation index of tumor cells in transitional area (ki-67 staining) In caspase-3, caspase-8 and Bcl-2 levels, the levels of caspase-3 and caspase-8 in stTRAIL-MSC were significantly higher than those in the other four groups, while the levels of Bcl-2 were lower than those in the other four groups RFA and blank control group analysis of the survival of rats found that RFA-treated rats were significantly longer survival time than the blank control group. CONCLUSIONS: StTRAIL-MSC targeting can promote the apoptosis of cancer cells in the RFA transitional region of hepatocellular carcinoma by increasing caspase cascade.