用两种抗粒细胞集落刺激因子（Ｇ－ＣＳＦ）单克隆抗体，本院自制了Ｇ－ＣＳＦ酶联免疫检测试剂盒。经国内７家大医院试用，对临床标本测定的结果表明：细菌培养阳性发热患者（ｎ＝３１），Ｇ－ＣＳＦ阳性检出率为１００％。未做细菌培养临床诊断为细菌感染发热患者（ｎ＝２６９），Ｇ－ＣＳＦ阳性率为８９．２％；非细菌感染发热患者（ｎ＝１５３），Ｇ－ＣＳＦ阳性率只有６．５％；正常人（ｎ＝１５７），Ｇ－ＣＳＦ检测全部为阴性。证实本方法可用于发热性疾病的诊断、鉴别诊断，并可指导临床抗生素的合理使用。 Two anti-granulocyte colony-stimulating factor (G-CSF) monoclonal antibodies, our hospital made a G-CSF ELISA kit. The results of the clinical specimen determination showed that in the seven major hospitals in China, the positive rate of G-CSF was 100% in patients with positive bacterial culture (n = 31). The positive rate of G-CSF was 89.2% in patients with fever without bacterial infection (n = 269). The positive rate of G-CSF in non-bacterial patients with fever was only 6.5% Normal (n = 157), G-CSF test all negative. Confirmed that the method can be used for the diagnosis of fever, differential diagnosis, and can guide the rational use of clinical antibiotics.